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KMID : 0043320010240060601
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Archives of Pharmacal Research
2001 Volume.24 No. 6 p.601 ~ p.606
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Determination of Recombinant Human Epidermal Growth factor (rhEGF) in a Pharmaceutical Preparation by Capillarr Electrophoresis
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Hwang KH
Lee KW/Kim CS/Han K/Chung YB/Moon DC
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Abstract
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A simple assay method of recombinant human epidermal growth factor (rhEGF) in a pharm- maceutical preparation was studied and validated by capillary electrophoresis (CE) using micellar electrokinetic chromatography (MIKC) techniques. Factors affecting the migration behavior and separation performances of the peptide; type of buffers Ph, buffer concentration, and concentration of sodium dodecyl sulfates (SDs) were investigated to optimize the analytical performance. CE was performed using running buffer, 50.0 Mm borate (Ph 8.5) containing 12.5 Mm SDs at 20 Kv of the applied voltage, Calibration cuties for the rhEGF showed good linearity (r>0.999) over the wide dynamic range from 1.25 to 100mcg/m1. Sample analysis was performed by using standard addition method to eliminate the matrix effects of dosage vehicle. This method is assumed to be useful for quality control (QC) of various forms of harmaceutical products of the peptide.
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